The SLUCare Flow Cytometry Laboratory offers a wide selection of comprehensive testing processes to provide the Hematopathology Service with the studies necessary to achieve excellence in diagnosis. The service uses state-of-the-art Beckman Coulter Navios EX Flow Cytometers, advanced Kaluza software and maintains more than 60 monoclonal antibodies, making it a major testing resource for the bi-state region. Antibodies employ different fluorescent probes to identify specific patterns of antigen expression. The laboratory offers 10-color flow cytometric analysis for neoplastic testing.
The SLUCare Flow Cytometry Laboratory offers specialized comprehensive studies for a variety of hematopoietic disorders:
- Immune deficiencies
- Lymphocyte T-cell subset evaluations
- CD34 stem cell enumeration
Hours of Operation
The Flow Cytometry Laboratory is open 8 a.m. to 5 p.m., Monday-Friday. Routine specimens should be delivered between 8 a.m. and 4 p.m., Monday-Friday. Emergency service after hours can be arranged at 314-977-7864.
Verbal reports are available within 24 to 48 hours. Final written reports will be faxed as soon as they are completed and are available online.
Request for Services
Specimens must be accompanied by a signed requisition and must comply with the labeling requirements of the Saint Louis University Department of Pathology and include a preliminary diagnosis so that the proper analysis will be performed. Samples must be delivered to:Hematopathology/Flow Cytometry Laboratory Schwitalla Hall, Room M468
3545 Vista - East of Grand
St. Louis, MO 63104
Body fluids (pleural, ascites, pericardial, cerebral spinal or CSF) and WASHINGS (fine needle aspirates or lung lavages) are acceptable for flow cytometric analysis if the cell number is sufficient. The minimal volume of sample required is 3 mls; however, depending upon cell number, this volume may not be sufficient for flow cytometric analysis. If the specimen is bloody, sodium heparin may be added to the specimen at collection. The sample should be kept at room temperature and delivered immediately to the laboratory. Receipt should be within 4 hours.
Processed samples of hematopoietic progenitor cell (HPC) harvests (apheresis, bone marrow) are acceptable for flow cytometric analysis of CD34+ stem cells. Samples should be sent at room temperature within 4 hours after processing.
To ensure that all specimens submitted to Flow Cytometry are received in good condition and within the appropriate time for testing, we request clients call the laboratory when a specimen is sent. The laboratory must be contacted if the specimen will arrive after hours or if there is a concern that the delivery of the specimen will not occur in the appropriate time frame.